Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei <i>in vitro</i> in Human Lymphocytes

Narinder Kumar Sharma

doi:doi:10.11648/j.ajbio.20150306.17

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Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes

Narinder Kumar Sharma

Published in American Journal of BioScience (Volume 3, Issue 6)

Received: 25 December 2014 Accepted: 5 January 2015 Published: 13 November 2015

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Abstract

Histamine H2 receptor antagonist are used in the clinical treatment of peptic ulcer. In vitro metaphase analysis and micronucleus assay were used to test the effect of Famotidine & Ranitidine on ⁶⁰Cobalt gamma-ray induced clastogenic effects. Heparinised whole blood was obtained from 6 healthy volunteers and was gamma irradiated with 3Gy. Lymphocyte cultures were initiated and aqueous solution of Famotidine (150µg/ml) & Ranitidine (500µg/ml) was added at 0h and 24h. Cultures were harvested & processed at 48h & 72h for chromosome aberrations and micronucleus analysis respectively. At 0h & 24h after 3Gy gamma irradiation, cultures treated with Famotidine & Ranitidine independently showed significant decrease (p < 0.0001) in the frequency of chromosome aberration. At 0h & 24h Famotidine induced 60.91% & 56.42% inhibition in dicentrics & 59.39% & 56.21% inhibition was observed in total aberrations where as Ranitidine induce 52.11% & 43.54% inhibition in dicentrics and 53.06% & 46.66% inhibition in total aberrations at 0h & 24h. Significant decrease in the frequency of micronuclei was observed with Famotidine treatment after 3Gy of gamma irradiation, which induced inhibition of 48.83% (p < 0.0001) at 0hr & 5.02% (p < 0.016) at 24h. However, Ranitidine induced significant decrease (p < 0.0001) in frequency of micronuclei of 28.85% at 0h where as a decrease in frequency was observed of 2.88% at 24h although not significant when compared with 3Gy gamma irradiation alone. In conclusion radio protective effects of Histamine H2 receptor antagonists Famotidine and Ranitidine was observed on exposure togamma-ray.

Published in	American Journal of BioScience (Volume 3, Issue 6)
DOI	10.11648/j.ajbio.20150306.17
Page(s)	249-255
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Ionizing Radiation, Radioprotection, Chromosomal Aberration, Cytochalasin-B Blocked Micronuclei, Histamine H2 Receptor Antagonist, Famotidine, Ranitidine, Human Lymphocytes

References

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Narinder Kumar Sharma. (2015). Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes. American Journal of BioScience, 3(6), 249-255. https://doi.org/10.11648/j.ajbio.20150306.17

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ACS Style

Narinder Kumar Sharma. Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes. Am. J. BioScience 2015, 3(6), 249-255. doi: 10.11648/j.ajbio.20150306.17

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AMA Style

Narinder Kumar Sharma. Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes. Am J BioScience. 2015;3(6):249-255. doi: 10.11648/j.ajbio.20150306.17

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@article{10.11648/j.ajbio.20150306.17,
  author = {Narinder Kumar Sharma},
  title = {Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {6},
  pages = {249-255},
  doi = {10.11648/j.ajbio.20150306.17},
  url = {https://doi.org/10.11648/j.ajbio.20150306.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150306.17},
  abstract = {Histamine H2 receptor antagonist are used in the clinical treatment of peptic ulcer. In vitro metaphase analysis and micronucleus assay were used to test the effect of Famotidine & Ranitidine on 60Cobalt gamma-ray induced clastogenic effects. Heparinised whole blood was obtained from 6 healthy volunteers and was gamma irradiated with 3Gy. Lymphocyte cultures were initiated and aqueous solution of Famotidine (150µg/ml) & Ranitidine (500µg/ml) was added at 0h and 24h. Cultures were harvested & processed at 48h & 72h for chromosome aberrations and micronucleus analysis respectively. At 0h & 24h after 3Gy gamma irradiation, cultures treated with Famotidine & Ranitidine independently showed significant decrease (p < 0.0001) in the frequency of chromosome aberration. At 0h & 24h Famotidine induced 60.91% & 56.42% inhibition in dicentrics & 59.39% & 56.21% inhibition was observed in total aberrations where as Ranitidine induce 52.11% & 43.54% inhibition in dicentrics and 53.06% & 46.66% inhibition in total aberrations at 0h & 24h. Significant decrease in the frequency of micronuclei was observed with Famotidine treatment after 3Gy of gamma irradiation, which induced inhibition of 48.83% (p < 0.0001) at 0hr & 5.02% (p < 0.016) at 24h. However, Ranitidine induced significant decrease (p < 0.0001) in frequency of micronuclei of 28.85% at 0h where as a decrease in frequency was observed of 2.88% at 24h although not significant when compared with 3Gy gamma irradiation alone. In conclusion radio protective effects of Histamine H2 receptor antagonists Famotidine and Ranitidine was observed on exposure togamma-ray.},
 year = {2015}
}

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TY  - JOUR
T1  - Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes
AU  - Narinder Kumar Sharma
Y1  - 2015/11/13
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.20150306.17
DO  - 10.11648/j.ajbio.20150306.17
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 249
EP  - 255
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20150306.17
AB  - Histamine H2 receptor antagonist are used in the clinical treatment of peptic ulcer. In vitro metaphase analysis and micronucleus assay were used to test the effect of Famotidine & Ranitidine on 60Cobalt gamma-ray induced clastogenic effects. Heparinised whole blood was obtained from 6 healthy volunteers and was gamma irradiated with 3Gy. Lymphocyte cultures were initiated and aqueous solution of Famotidine (150µg/ml) & Ranitidine (500µg/ml) was added at 0h and 24h. Cultures were harvested & processed at 48h & 72h for chromosome aberrations and micronucleus analysis respectively. At 0h & 24h after 3Gy gamma irradiation, cultures treated with Famotidine & Ranitidine independently showed significant decrease (p < 0.0001) in the frequency of chromosome aberration. At 0h & 24h Famotidine induced 60.91% & 56.42% inhibition in dicentrics & 59.39% & 56.21% inhibition was observed in total aberrations where as Ranitidine induce 52.11% & 43.54% inhibition in dicentrics and 53.06% & 46.66% inhibition in total aberrations at 0h & 24h. Significant decrease in the frequency of micronuclei was observed with Famotidine treatment after 3Gy of gamma irradiation, which induced inhibition of 48.83% (p < 0.0001) at 0hr & 5.02% (p < 0.016) at 24h. However, Ranitidine induced significant decrease (p < 0.0001) in frequency of micronuclei of 28.85% at 0h where as a decrease in frequency was observed of 2.88% at 24h although not significant when compared with 3Gy gamma irradiation alone. In conclusion radio protective effects of Histamine H2 receptor antagonists Famotidine and Ranitidine was observed on exposure togamma-ray.
VL  - 3
IS  - 6
ER  -

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Author Information

Narinder Kumar Sharma

Radiation Biology and Health Sciences Division, Bio-Sciences Group, Bhabha Atomic Research Centre, Mumbai, India

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Narinder Kumar Sharma. (2015). Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes. American Journal of BioScience, 3(6), 249-255. https://doi.org/10.11648/j.ajbio.20150306.17

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ACS Style

Narinder Kumar Sharma. Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes. Am. J. BioScience 2015, 3(6), 249-255. doi: 10.11648/j.ajbio.20150306.17

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AMA Style

Narinder Kumar Sharma. Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes. Am J BioScience. 2015;3(6):249-255. doi: 10.11648/j.ajbio.20150306.17

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@article{10.11648/j.ajbio.20150306.17,
  author = {Narinder Kumar Sharma},
  title = {Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {6},
  pages = {249-255},
  doi = {10.11648/j.ajbio.20150306.17},
  url = {https://doi.org/10.11648/j.ajbio.20150306.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150306.17},
  abstract = {Histamine H2 receptor antagonist are used in the clinical treatment of peptic ulcer. In vitro metaphase analysis and micronucleus assay were used to test the effect of Famotidine & Ranitidine on 60Cobalt gamma-ray induced clastogenic effects. Heparinised whole blood was obtained from 6 healthy volunteers and was gamma irradiated with 3Gy. Lymphocyte cultures were initiated and aqueous solution of Famotidine (150µg/ml) & Ranitidine (500µg/ml) was added at 0h and 24h. Cultures were harvested & processed at 48h & 72h for chromosome aberrations and micronucleus analysis respectively. At 0h & 24h after 3Gy gamma irradiation, cultures treated with Famotidine & Ranitidine independently showed significant decrease (p < 0.0001) in the frequency of chromosome aberration. At 0h & 24h Famotidine induced 60.91% & 56.42% inhibition in dicentrics & 59.39% & 56.21% inhibition was observed in total aberrations where as Ranitidine induce 52.11% & 43.54% inhibition in dicentrics and 53.06% & 46.66% inhibition in total aberrations at 0h & 24h. Significant decrease in the frequency of micronuclei was observed with Famotidine treatment after 3Gy of gamma irradiation, which induced inhibition of 48.83% (p < 0.0001) at 0hr & 5.02% (p < 0.016) at 24h. However, Ranitidine induced significant decrease (p < 0.0001) in frequency of micronuclei of 28.85% at 0h where as a decrease in frequency was observed of 2.88% at 24h although not significant when compared with 3Gy gamma irradiation alone. In conclusion radio protective effects of Histamine H2 receptor antagonists Famotidine and Ranitidine was observed on exposure togamma-ray.},
 year = {2015}
}

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TY  - JOUR
T1  - Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes
AU  - Narinder Kumar Sharma
Y1  - 2015/11/13
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.20150306.17
DO  - 10.11648/j.ajbio.20150306.17
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 249
EP  - 255
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20150306.17
AB  - Histamine H2 receptor antagonist are used in the clinical treatment of peptic ulcer. In vitro metaphase analysis and micronucleus assay were used to test the effect of Famotidine & Ranitidine on 60Cobalt gamma-ray induced clastogenic effects. Heparinised whole blood was obtained from 6 healthy volunteers and was gamma irradiated with 3Gy. Lymphocyte cultures were initiated and aqueous solution of Famotidine (150µg/ml) & Ranitidine (500µg/ml) was added at 0h and 24h. Cultures were harvested & processed at 48h & 72h for chromosome aberrations and micronucleus analysis respectively. At 0h & 24h after 3Gy gamma irradiation, cultures treated with Famotidine & Ranitidine independently showed significant decrease (p < 0.0001) in the frequency of chromosome aberration. At 0h & 24h Famotidine induced 60.91% & 56.42% inhibition in dicentrics & 59.39% & 56.21% inhibition was observed in total aberrations where as Ranitidine induce 52.11% & 43.54% inhibition in dicentrics and 53.06% & 46.66% inhibition in total aberrations at 0h & 24h. Significant decrease in the frequency of micronuclei was observed with Famotidine treatment after 3Gy of gamma irradiation, which induced inhibition of 48.83% (p < 0.0001) at 0hr & 5.02% (p < 0.016) at 24h. However, Ranitidine induced significant decrease (p < 0.0001) in frequency of micronuclei of 28.85% at 0h where as a decrease in frequency was observed of 2.88% at 24h although not significant when compared with 3Gy gamma irradiation alone. In conclusion radio protective effects of Histamine H2 receptor antagonists Famotidine and Ranitidine was observed on exposure togamma-ray.
VL  - 3
IS  - 6
ER  -

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