Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia

Gadhia Pankaj K.; Patel Monika V.; Vaniawala Salil N.

doi:doi:10.11648/j.ajbls.20160406.13

| Peer-Reviewed

Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia

Gadhia Pankaj K., Patel Monika V., Vaniawala Salil N.

Published in American Journal of Biomedical and Life Sciences (Volume 4, Issue 6)

Received: 21 September 2016 Accepted: 11 November 2016 Published: 14 December 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Aim: Acute leukaemia represents clonal haematological disorders that arise from at least two or more genetics alteration in susceptible haematological cells. The cytogenetic study confirms a wide variety of common, rare and novel chromosomal anomalies in patients with haematological disorders providing valuable diagnostics and prognostic information. Method: Cytogenetic analyses were carried out in a total 4600 suspected patients. Of which, 68 patients were reported with Acute Myeloid Leukaemia. Cytogenetic analyses from bone marrow cultures having age ranging from 5 years to 65 years were carried out. GTG banded metaphases were analysed and karyotypes by automatic karyotyping system and confirmation were made by using Florescent In Situ Hybridization technique (FISH). Results: Results revealed that out of 68 AML patients only 36 patients (52.9%) were found with translocation t(8; 21) (q22; q22) in AML-M2 subtype, 23 patients (33.8%) were found with a translocation t(15; 17) (q22; q12) in AML-M3 and only 09 patients(13.2%) were found with inversion in chromosome16 inv(16) (p13; q22) in AML-M4. Conclusion: It is concluded from the present study that a high prevalence rate of AML were found in t(8; 21) (q22; q22) followed by t(15; 17) (q22; q12) and inv(16) (p13; q22). The significance of results is discussed.

Published in	American Journal of Biomedical and Life Sciences (Volume 4, Issue 6)
DOI	10.11648/j.ajbls.20160406.13
Page(s)	98-102
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

G-banding, Karyotype, AML, FISH

References

[1]	SL. Starza, C. Matteucci, B. Crescenzi, G. Perla, M. Carotenuto, MF. Martelli et al., “Identification of chromosome changes in Acute myeloid leukemia (AML-L2) by Molecular cytogenetics” Can Genet Cytogenet, 1997, 95, pp. 148-152.
[2]	S Yohe, “ Molecular Genetic markers in Acute leukemia”. J. Clin. Med., 2015, 4: pp. 460-478.
[3]	S. Frohling, RF. Schlenk, J. Breitruck, A. Benner, KS. Tobis, H. Dohner and K. Dohner, “Prognostic significance of activating FLT3 mutations in younger adults (16 to 60 years) with acute myeloid leukemia and normal cytogenetics: a study of AML study Group Ulm”, Blood, 2002, 100: pp. 4372-4380.
[4]	O. Blau, R. Berenstein, A. Sindram, IW. Blau. “Molecular analysis of different FLT3-ITD mutations in acute myeloid leukemia”. Leuk. Lymphoma 2013, 54, pp. 145–152.
[5]	TL. Lin, T. Williams, J. He, OS. Aljitawi, S. Ganguly, S. Abhyankar, S. Fleming, H. Male and JP. McGuirk, “Rates of complete diagnostic testing for patients with acute myeloid leukemia” Cancer Mdicine, 2015, 4(4): pp. 519-522.
[6]	ED. Velloso, CH, Motta, JB. Furtado, NS. Bacal, PA. Silveira, CB. Moyses, R. Sitnik, and JR. Pinho, “Molecular and cytogenetic abnormalities in acute myeloid leukemia: Review and case studies”, Einstein, 2011, 9(2), pp. 184-189.
[7]	F. Ahmed, P. Kokte, P. Chedda, R. Dalvi, B. Ranjandas and S. Mandava, “Molecular cytogenetic findings in a three-way novel variant of t(1; 8; 21) (p35; q22; q22) a unique relocation of the AML1/ETO fusion gene 1p35 in AML-M2”, Cancer Genet. Cytogenet., 2008, 180: pp. 153-157.
[8]	ISCN, International System for Human Cytogenetics Nomenclature (ISCN), 2009, S. Karger Publ. Inc.
[9]	RM, Stone, MR, O’Donnell, MA, Sekeers, “Acute myeloid leukemia”, Am. Soc. Hematol. Edu. Program, 2004, pp. 98-117.
[10]	B, Williams, A, Amon, (2009). Aneuploidy: Cancer's Fatal Flaw?. Cancer Research, 69(13), pp. 5289-5291.
[11]	D, Grimwade, RK, Hills, AV, Moorman, H, Walker S, Chatters, AH, Goldstone, AK, Burnett, “Refinement of cytogenetic classification in acute myeloid leukaemia: determination of prognostic significance of rare recurring chromosomal abnormalities among 5876 younger adult patients treated in the United Kingdom Medical Research Council Trials” Blood, 116(3), pp 354-365.
[12]	C. Chandra Kumar, “Genetic abnormality and challenges in the treatment of Acute myeloid leukemia”, Gene and Cancer, 2011, 2(2): pp. 95-107.
[13]	MLLF. Chauffaile, MS. Figueiredo, R. Beltrani, R. Antunes, SV. Yamomoto and J. Kerbauy, “Acute promyelocytic leukemia: the study of t(15; 17) transloction by fluorescent in situ hybridization, reverse trancriptase polymerase chain reaction and cytogenetic techniques” Brazilian J. Medical and Biological Research, 2001, 34: pp. 735-743.

Cite This Article

Plain Text BibTeX RIS

APA Style

Gadhia Pankaj K., Patel Monika V., Vaniawala Salil N. (2016). Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia. American Journal of Biomedical and Life Sciences, 4(6), 98-102. https://doi.org/10.11648/j.ajbls.20160406.13

Copy | Download

ACS Style

Gadhia Pankaj K.; Patel Monika V.; Vaniawala Salil N. Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia. Am. J. Biomed. Life Sci. 2016, 4(6), 98-102. doi: 10.11648/j.ajbls.20160406.13

Copy | Download

AMA Style

Gadhia Pankaj K., Patel Monika V., Vaniawala Salil N. Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia. Am J Biomed Life Sci. 2016;4(6):98-102. doi: 10.11648/j.ajbls.20160406.13

Copy | Download

@article{10.11648/j.ajbls.20160406.13,
  author = {Gadhia Pankaj K. and Patel Monika V. and Vaniawala Salil N.},
  title = {Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {6},
  pages = {98-102},
  doi = {10.11648/j.ajbls.20160406.13},
  url = {https://doi.org/10.11648/j.ajbls.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160406.13},
  abstract = {Aim: Acute leukaemia represents clonal haematological disorders that arise from at least two or more genetics alteration in susceptible haematological cells. The cytogenetic study confirms a wide variety of common, rare and novel chromosomal anomalies in patients with haematological disorders providing valuable diagnostics and prognostic information. Method: Cytogenetic analyses were carried out in a total 4600 suspected patients. Of which, 68 patients were reported with Acute Myeloid Leukaemia. Cytogenetic analyses from bone marrow cultures having age ranging from 5 years to 65 years were carried out. GTG banded metaphases were analysed and karyotypes by automatic karyotyping system and confirmation were made by using Florescent In Situ Hybridization technique (FISH). Results: Results revealed that out of 68 AML patients only 36 patients (52.9%) were found with translocation t(8; 21) (q22; q22) in AML-M2 subtype, 23 patients (33.8%) were found with a translocation t(15; 17) (q22; q12) in AML-M3 and only 09 patients(13.2%) were found with inversion in chromosome16 inv(16) (p13; q22) in AML-M4. Conclusion: It is concluded from the present study that a high prevalence rate of AML were found in t(8; 21) (q22; q22) followed by t(15; 17) (q22; q12) and inv(16) (p13; q22). The significance of results is discussed.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia
AU  - Gadhia Pankaj K.
AU  - Patel Monika V.
AU  - Vaniawala Salil N.
Y1  - 2016/12/14
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20160406.13
DO  - 10.11648/j.ajbls.20160406.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 98
EP  - 102
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20160406.13
AB  - Aim: Acute leukaemia represents clonal haematological disorders that arise from at least two or more genetics alteration in susceptible haematological cells. The cytogenetic study confirms a wide variety of common, rare and novel chromosomal anomalies in patients with haematological disorders providing valuable diagnostics and prognostic information. Method: Cytogenetic analyses were carried out in a total 4600 suspected patients. Of which, 68 patients were reported with Acute Myeloid Leukaemia. Cytogenetic analyses from bone marrow cultures having age ranging from 5 years to 65 years were carried out. GTG banded metaphases were analysed and karyotypes by automatic karyotyping system and confirmation were made by using Florescent In Situ Hybridization technique (FISH). Results: Results revealed that out of 68 AML patients only 36 patients (52.9%) were found with translocation t(8; 21) (q22; q22) in AML-M2 subtype, 23 patients (33.8%) were found with a translocation t(15; 17) (q22; q12) in AML-M3 and only 09 patients(13.2%) were found with inversion in chromosome16 inv(16) (p13; q22) in AML-M4. Conclusion: It is concluded from the present study that a high prevalence rate of AML were found in t(8; 21) (q22; q22) followed by t(15; 17) (q22; q12) and inv(16) (p13; q22). The significance of results is discussed.
VL  - 4
IS  - 6
ER  -

Copy | Download

Author Information

Gadhia Pankaj K.

Cytogenetic Unit, S. N. Gene Laboratory and Research Centre, Surat, India
Patel Monika V.

Cytogenetic Unit, S. N. Gene Laboratory and Research Centre, Surat, India
Vaniawala Salil N.

Cytogenetic Unit, S. N. Gene Laboratory and Research Centre, Surat, India

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Gadhia Pankaj K., Patel Monika V., Vaniawala Salil N. (2016). Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia. American Journal of Biomedical and Life Sciences, 4(6), 98-102. https://doi.org/10.11648/j.ajbls.20160406.13

Copy | Download

ACS Style

Gadhia Pankaj K.; Patel Monika V.; Vaniawala Salil N. Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia. Am. J. Biomed. Life Sci. 2016, 4(6), 98-102. doi: 10.11648/j.ajbls.20160406.13

Copy | Download

AMA Style

Gadhia Pankaj K., Patel Monika V., Vaniawala Salil N. Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia. Am J Biomed Life Sci. 2016;4(6):98-102. doi: 10.11648/j.ajbls.20160406.13

Copy | Download

@article{10.11648/j.ajbls.20160406.13,
  author = {Gadhia Pankaj K. and Patel Monika V. and Vaniawala Salil N.},
  title = {Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {6},
  pages = {98-102},
  doi = {10.11648/j.ajbls.20160406.13},
  url = {https://doi.org/10.11648/j.ajbls.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160406.13},
  abstract = {Aim: Acute leukaemia represents clonal haematological disorders that arise from at least two or more genetics alteration in susceptible haematological cells. The cytogenetic study confirms a wide variety of common, rare and novel chromosomal anomalies in patients with haematological disorders providing valuable diagnostics and prognostic information. Method: Cytogenetic analyses were carried out in a total 4600 suspected patients. Of which, 68 patients were reported with Acute Myeloid Leukaemia. Cytogenetic analyses from bone marrow cultures having age ranging from 5 years to 65 years were carried out. GTG banded metaphases were analysed and karyotypes by automatic karyotyping system and confirmation were made by using Florescent In Situ Hybridization technique (FISH). Results: Results revealed that out of 68 AML patients only 36 patients (52.9%) were found with translocation t(8; 21) (q22; q22) in AML-M2 subtype, 23 patients (33.8%) were found with a translocation t(15; 17) (q22; q12) in AML-M3 and only 09 patients(13.2%) were found with inversion in chromosome16 inv(16) (p13; q22) in AML-M4. Conclusion: It is concluded from the present study that a high prevalence rate of AML were found in t(8; 21) (q22; q22) followed by t(15; 17) (q22; q12) and inv(16) (p13; q22). The significance of results is discussed.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Role of Cytogenetic Evaluation in Diagnosis of Acute Myeloid Leukemia
AU  - Gadhia Pankaj K.
AU  - Patel Monika V.
AU  - Vaniawala Salil N.
Y1  - 2016/12/14
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20160406.13
DO  - 10.11648/j.ajbls.20160406.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 98
EP  - 102
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20160406.13
AB  - Aim: Acute leukaemia represents clonal haematological disorders that arise from at least two or more genetics alteration in susceptible haematological cells. The cytogenetic study confirms a wide variety of common, rare and novel chromosomal anomalies in patients with haematological disorders providing valuable diagnostics and prognostic information. Method: Cytogenetic analyses were carried out in a total 4600 suspected patients. Of which, 68 patients were reported with Acute Myeloid Leukaemia. Cytogenetic analyses from bone marrow cultures having age ranging from 5 years to 65 years were carried out. GTG banded metaphases were analysed and karyotypes by automatic karyotyping system and confirmation were made by using Florescent In Situ Hybridization technique (FISH). Results: Results revealed that out of 68 AML patients only 36 patients (52.9%) were found with translocation t(8; 21) (q22; q22) in AML-M2 subtype, 23 patients (33.8%) were found with a translocation t(15; 17) (q22; q12) in AML-M3 and only 09 patients(13.2%) were found with inversion in chromosome16 inv(16) (p13; q22) in AML-M4. Conclusion: It is concluded from the present study that a high prevalence rate of AML were found in t(8; 21) (q22; q22) followed by t(15; 17) (q22; q12) and inv(16) (p13; q22). The significance of results is discussed.
VL  - 4
IS  - 6
ER  -

Copy | Download