Leptospirosis is a zoonotic infection, which occurs mostly in persons venturing in aquatic environment, like farmers and sanitary workers. The objectives of the study were to isolate leptospires and to screen for leptospiral antibodies by MAT and IgM and IgG plate ELISAs to ascertain leptospirosis among suspected leptospirosis cases in and around Chennai. 198 cases of suspected leptospirosis cases attending government and private hospitals in Chennai, were included for this study. Blood samples collected in triplicate at an interval of 7 days from each case, were subjected to culture and leptospiral antibody detection by MAT and IgM and IgG plate ELISAs. The corresponding urine samples were screened for culture only. Out of the 198 cases, 118 (59.5%) were positive by MAT and 124 (62.6%) were positive by IgM and IgG plate ELISAs. Only two isolates of leptospira were possible, that too in urine only. One of the isolates belonged to serogroup Canicola and the other to Autumnalis. To conclude, leptospirosis is endemic in and around Chennai and so proper identification, diagnosis and treatment, apart from prophylactic measures are required.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 2, Issue 4) |
| DOI | 10.11648/j.ajcem.20140204.13 |
| Page(s) | 70-73 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Leptospirosis, Isolation, Canicola, Autumnalis, MAT, Plate ELISA
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APA Style
L. Suresh Babu. (2014). Isolation and Serological Evidences in Suspected Leptospirosis Cases in and Around Chennai. American Journal of Clinical and Experimental Medicine, 2(4), 70-73. https://doi.org/10.11648/j.ajcem.20140204.13
ACS Style
L. Suresh Babu. Isolation and Serological Evidences in Suspected Leptospirosis Cases in and Around Chennai. Am. J. Clin. Exp. Med. 2014, 2(4), 70-73. doi: 10.11648/j.ajcem.20140204.13
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Download@article{10.11648/j.ajcem.20140204.13,
author = {L. Suresh Babu},
title = {Isolation and Serological Evidences in Suspected Leptospirosis Cases in and Around Chennai},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {2},
number = {4},
pages = {70-73},
doi = {10.11648/j.ajcem.20140204.13},
url = {https://doi.org/10.11648/j.ajcem.20140204.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140204.13},
abstract = {Leptospirosis is a zoonotic infection, which occurs mostly in persons venturing in aquatic environment, like farmers and sanitary workers. The objectives of the study were to isolate leptospires and to screen for leptospiral antibodies by MAT and IgM and IgG plate ELISAs to ascertain leptospirosis among suspected leptospirosis cases in and around Chennai. 198 cases of suspected leptospirosis cases attending government and private hospitals in Chennai, were included for this study. Blood samples collected in triplicate at an interval of 7 days from each case, were subjected to culture and leptospiral antibody detection by MAT and IgM and IgG plate ELISAs. The corresponding urine samples were screened for culture only. Out of the 198 cases, 118 (59.5%) were positive by MAT and 124 (62.6%) were positive by IgM and IgG plate ELISAs. Only two isolates of leptospira were possible, that too in urine only. One of the isolates belonged to serogroup Canicola and the other to Autumnalis. To conclude, leptospirosis is endemic in and around Chennai and so proper identification, diagnosis and treatment, apart from prophylactic measures are required.},
year = {2014}
}
TY - JOUR T1 - Isolation and Serological Evidences in Suspected Leptospirosis Cases in and Around Chennai AU - L. Suresh Babu Y1 - 2014/07/20 PY - 2014 N1 - https://doi.org/10.11648/j.ajcem.20140204.13 DO - 10.11648/j.ajcem.20140204.13 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 70 EP - 73 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20140204.13 AB - Leptospirosis is a zoonotic infection, which occurs mostly in persons venturing in aquatic environment, like farmers and sanitary workers. The objectives of the study were to isolate leptospires and to screen for leptospiral antibodies by MAT and IgM and IgG plate ELISAs to ascertain leptospirosis among suspected leptospirosis cases in and around Chennai. 198 cases of suspected leptospirosis cases attending government and private hospitals in Chennai, were included for this study. Blood samples collected in triplicate at an interval of 7 days from each case, were subjected to culture and leptospiral antibody detection by MAT and IgM and IgG plate ELISAs. The corresponding urine samples were screened for culture only. Out of the 198 cases, 118 (59.5%) were positive by MAT and 124 (62.6%) were positive by IgM and IgG plate ELISAs. Only two isolates of leptospira were possible, that too in urine only. One of the isolates belonged to serogroup Canicola and the other to Autumnalis. To conclude, leptospirosis is endemic in and around Chennai and so proper identification, diagnosis and treatment, apart from prophylactic measures are required. VL - 2 IS - 4 ER -
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