Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of <i>Eucalyptus saligna</i> on <i>Sitophilus zeamais</i> Motschulsky, 1855 (Coleoptera: Curculionidae)

Timothy Omara; Fredrick Kizza Kateeba; Bashir Musau; Erisa Kigenyi; Eddie Adupa; Sarah Kagoya

doi:doi:10.11648/j.jher.20180404.15

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Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae)

Timothy Omara, Fredrick Kizza Kateeba, Bashir Musau, Erisa Kigenyi, Eddie Adupa, Sarah Kagoya

Published in Journal of Health and Environmental Research (Volume 4, Issue 4)

Received: 10 November 2018 Accepted: 9 December 2018 Published: 22 January 2019

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Abstract

Exploration of plants, animals, microbes and their products for green pesticides have been the mainstay of modern agriculturalists. Plant bioactive oils have been utilized in formulation of green pesticides, which are less toxic, least deleterious on biocontrol agents, readily biodegraded and have no or few ecological effects as compared to synthetic equivalents. This study evaluated the bioinsecticidal activity of Eucalyptol and 1R-Alpha-Pinene rich acetonic essential oils of dry leaves of the Sydney Blue gum (Eucalyptus saligna Smith) on common granivorous maize weevil. Fresh leaves of Eucalyptus saligna were harvested and the essential oils extracted from its dry powder by hydrodistillation. The chemical composition of the essential oil was analyzed by tandem GC/MS. Twelve components were identified, and the main components were Eucalyptol (34.36%) and 1R-alpha pinene (17.92%). Acetonic essential oils of 2µL, 4µL, 6µL and 8µL in 1ml acetone were used in contact toxicity, fumigant and repellent bioassays. In contact toxicity, 8µL/ml oil exerted a rapid effect on the weevils giving 100% mortality in the first day of exposure; 6µL/ml achieved 100% mortality 96 hours after treatment. Both fumigant and repellent activities showed a gradual increase in insect mortality and repellency with time of exposure as the concentrations of the essential oils were increased; 100% repellency was achieved at 8µL in the second hour and this remained constant with time. The lowest repellency of 20% was obtained at 2µL. In fumigant toxicity, the highest mortality was 70% recorded at 8µL on the third day of exposure. The results indicated that the acetonic essential oil of dry Eucalyptol and 1R-Alpha-Pinene rich Eucalyptus saligna leaves can be developed into a bioinsecticide for controlling maize weevil as a repellent, toxicant and fumigant candidate.

Published in	Journal of Health and Environmental Research (Volume 4, Issue 4)
DOI	10.11648/j.jher.20180404.15
Page(s)	153-160
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Insecticide, Bioactive, Fumigant, Contact Toxicity, Repellent, Extraction

References

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Timothy Omara, Fredrick Kizza Kateeba, Bashir Musau, Erisa Kigenyi, Eddie Adupa, et al. (2019). Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae). Journal of Health and Environmental Research, 4(4), 153-160. https://doi.org/10.11648/j.jher.20180404.15

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ACS Style

Timothy Omara; Fredrick Kizza Kateeba; Bashir Musau; Erisa Kigenyi; Eddie Adupa, et al. Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae). J. Health Environ. Res. 2019, 4(4), 153-160. doi: 10.11648/j.jher.20180404.15

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Timothy Omara, Fredrick Kizza Kateeba, Bashir Musau, Erisa Kigenyi, Eddie Adupa, et al. Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae). J Health Environ Res. 2019;4(4):153-160. doi: 10.11648/j.jher.20180404.15

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@article{10.11648/j.jher.20180404.15,
  author = {Timothy Omara and Fredrick Kizza Kateeba and Bashir Musau and Erisa Kigenyi and Eddie Adupa and Sarah Kagoya},
  title = {Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae)},
  journal = {Journal of Health and Environmental Research},
  volume = {4},
  number = {4},
  pages = {153-160},
  doi = {10.11648/j.jher.20180404.15},
  url = {https://doi.org/10.11648/j.jher.20180404.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20180404.15},
  abstract = {Exploration of plants, animals, microbes and their products for green pesticides have been the mainstay of modern agriculturalists. Plant bioactive oils have been utilized in formulation of green pesticides, which are less toxic, least deleterious on biocontrol agents, readily biodegraded and have no or few ecological effects as compared to synthetic equivalents. This study evaluated the bioinsecticidal activity of Eucalyptol and 1R-Alpha-Pinene rich acetonic essential oils of dry leaves of the Sydney Blue gum (Eucalyptus saligna Smith) on common granivorous maize weevil. Fresh leaves of Eucalyptus saligna were harvested and the essential oils extracted from its dry powder by hydrodistillation. The chemical composition of the essential oil was analyzed by tandem GC/MS. Twelve components were identified, and the main components were Eucalyptol (34.36%) and 1R-alpha pinene (17.92%). Acetonic essential oils of 2µL, 4µL, 6µL and 8µL in 1ml acetone were used in contact toxicity, fumigant and repellent bioassays. In contact toxicity, 8µL/ml oil exerted a rapid effect on the weevils giving 100% mortality in the first day of exposure; 6µL/ml achieved 100% mortality 96 hours after treatment. Both fumigant and repellent activities showed a gradual increase in insect mortality and repellency with time of exposure as the concentrations of the essential oils were increased; 100% repellency was achieved at 8µL in the second hour and this remained constant with time. The lowest repellency of 20% was obtained at 2µL. In fumigant toxicity, the highest mortality was 70% recorded at 8µL on the third day of exposure. The results indicated that the acetonic essential oil of dry Eucalyptol and 1R-Alpha-Pinene rich Eucalyptus saligna leaves can be developed into a bioinsecticide for controlling maize weevil as a repellent, toxicant and fumigant candidate.},
 year = {2019}
}

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TY  - JOUR
T1  - Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae)
AU  - Timothy Omara
AU  - Fredrick Kizza Kateeba
AU  - Bashir Musau
AU  - Erisa Kigenyi
AU  - Eddie Adupa
AU  - Sarah Kagoya
Y1  - 2019/01/22
PY  - 2019
N1  - https://doi.org/10.11648/j.jher.20180404.15
DO  - 10.11648/j.jher.20180404.15
T2  - Journal of Health and Environmental Research
JF  - Journal of Health and Environmental Research
JO  - Journal of Health and Environmental Research
SP  - 153
EP  - 160
PB  - Science Publishing Group
SN  - 2472-3592
UR  - https://doi.org/10.11648/j.jher.20180404.15
AB  - Exploration of plants, animals, microbes and their products for green pesticides have been the mainstay of modern agriculturalists. Plant bioactive oils have been utilized in formulation of green pesticides, which are less toxic, least deleterious on biocontrol agents, readily biodegraded and have no or few ecological effects as compared to synthetic equivalents. This study evaluated the bioinsecticidal activity of Eucalyptol and 1R-Alpha-Pinene rich acetonic essential oils of dry leaves of the Sydney Blue gum (Eucalyptus saligna Smith) on common granivorous maize weevil. Fresh leaves of Eucalyptus saligna were harvested and the essential oils extracted from its dry powder by hydrodistillation. The chemical composition of the essential oil was analyzed by tandem GC/MS. Twelve components were identified, and the main components were Eucalyptol (34.36%) and 1R-alpha pinene (17.92%). Acetonic essential oils of 2µL, 4µL, 6µL and 8µL in 1ml acetone were used in contact toxicity, fumigant and repellent bioassays. In contact toxicity, 8µL/ml oil exerted a rapid effect on the weevils giving 100% mortality in the first day of exposure; 6µL/ml achieved 100% mortality 96 hours after treatment. Both fumigant and repellent activities showed a gradual increase in insect mortality and repellency with time of exposure as the concentrations of the essential oils were increased; 100% repellency was achieved at 8µL in the second hour and this remained constant with time. The lowest repellency of 20% was obtained at 2µL. In fumigant toxicity, the highest mortality was 70% recorded at 8µL on the third day of exposure. The results indicated that the acetonic essential oil of dry Eucalyptol and 1R-Alpha-Pinene rich Eucalyptus saligna leaves can be developed into a bioinsecticide for controlling maize weevil as a repellent, toxicant and fumigant candidate.
VL  - 4
IS  - 4
ER  -

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Author Information

Timothy Omara

Department of Health Sciences, Unicaf University, Lusaka, Zambia
Fredrick Kizza Kateeba

Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda
Bashir Musau

Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda
Erisa Kigenyi

Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda
Eddie Adupa

Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda
Sarah Kagoya

Department of Chemistry, Faculty of Science, Kyambogo University, Kampala, Uganda

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Timothy Omara, Fredrick Kizza Kateeba, Bashir Musau, Erisa Kigenyi, Eddie Adupa, et al. (2019). Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae). Journal of Health and Environmental Research, 4(4), 153-160. https://doi.org/10.11648/j.jher.20180404.15

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ACS Style

Timothy Omara; Fredrick Kizza Kateeba; Bashir Musau; Erisa Kigenyi; Eddie Adupa, et al. Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae). J. Health Environ. Res. 2019, 4(4), 153-160. doi: 10.11648/j.jher.20180404.15

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Timothy Omara, Fredrick Kizza Kateeba, Bashir Musau, Erisa Kigenyi, Eddie Adupa, et al. Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae). J Health Environ Res. 2019;4(4):153-160. doi: 10.11648/j.jher.20180404.15

Copy | Download

@article{10.11648/j.jher.20180404.15,
  author = {Timothy Omara and Fredrick Kizza Kateeba and Bashir Musau and Erisa Kigenyi and Eddie Adupa and Sarah Kagoya},
  title = {Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae)},
  journal = {Journal of Health and Environmental Research},
  volume = {4},
  number = {4},
  pages = {153-160},
  doi = {10.11648/j.jher.20180404.15},
  url = {https://doi.org/10.11648/j.jher.20180404.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20180404.15},
  abstract = {Exploration of plants, animals, microbes and their products for green pesticides have been the mainstay of modern agriculturalists. Plant bioactive oils have been utilized in formulation of green pesticides, which are less toxic, least deleterious on biocontrol agents, readily biodegraded and have no or few ecological effects as compared to synthetic equivalents. This study evaluated the bioinsecticidal activity of Eucalyptol and 1R-Alpha-Pinene rich acetonic essential oils of dry leaves of the Sydney Blue gum (Eucalyptus saligna Smith) on common granivorous maize weevil. Fresh leaves of Eucalyptus saligna were harvested and the essential oils extracted from its dry powder by hydrodistillation. The chemical composition of the essential oil was analyzed by tandem GC/MS. Twelve components were identified, and the main components were Eucalyptol (34.36%) and 1R-alpha pinene (17.92%). Acetonic essential oils of 2µL, 4µL, 6µL and 8µL in 1ml acetone were used in contact toxicity, fumigant and repellent bioassays. In contact toxicity, 8µL/ml oil exerted a rapid effect on the weevils giving 100% mortality in the first day of exposure; 6µL/ml achieved 100% mortality 96 hours after treatment. Both fumigant and repellent activities showed a gradual increase in insect mortality and repellency with time of exposure as the concentrations of the essential oils were increased; 100% repellency was achieved at 8µL in the second hour and this remained constant with time. The lowest repellency of 20% was obtained at 2µL. In fumigant toxicity, the highest mortality was 70% recorded at 8µL on the third day of exposure. The results indicated that the acetonic essential oil of dry Eucalyptol and 1R-Alpha-Pinene rich Eucalyptus saligna leaves can be developed into a bioinsecticide for controlling maize weevil as a repellent, toxicant and fumigant candidate.},
 year = {2019}
}

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TY  - JOUR
T1  - Bioinsecticidal Activity of Eucalyptol and 1R-Alpha-Pinene Rich Acetonic Oils of Eucalyptus saligna on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae)
AU  - Timothy Omara
AU  - Fredrick Kizza Kateeba
AU  - Bashir Musau
AU  - Erisa Kigenyi
AU  - Eddie Adupa
AU  - Sarah Kagoya
Y1  - 2019/01/22
PY  - 2019
N1  - https://doi.org/10.11648/j.jher.20180404.15
DO  - 10.11648/j.jher.20180404.15
T2  - Journal of Health and Environmental Research
JF  - Journal of Health and Environmental Research
JO  - Journal of Health and Environmental Research
SP  - 153
EP  - 160
PB  - Science Publishing Group
SN  - 2472-3592
UR  - https://doi.org/10.11648/j.jher.20180404.15
AB  - Exploration of plants, animals, microbes and their products for green pesticides have been the mainstay of modern agriculturalists. Plant bioactive oils have been utilized in formulation of green pesticides, which are less toxic, least deleterious on biocontrol agents, readily biodegraded and have no or few ecological effects as compared to synthetic equivalents. This study evaluated the bioinsecticidal activity of Eucalyptol and 1R-Alpha-Pinene rich acetonic essential oils of dry leaves of the Sydney Blue gum (Eucalyptus saligna Smith) on common granivorous maize weevil. Fresh leaves of Eucalyptus saligna were harvested and the essential oils extracted from its dry powder by hydrodistillation. The chemical composition of the essential oil was analyzed by tandem GC/MS. Twelve components were identified, and the main components were Eucalyptol (34.36%) and 1R-alpha pinene (17.92%). Acetonic essential oils of 2µL, 4µL, 6µL and 8µL in 1ml acetone were used in contact toxicity, fumigant and repellent bioassays. In contact toxicity, 8µL/ml oil exerted a rapid effect on the weevils giving 100% mortality in the first day of exposure; 6µL/ml achieved 100% mortality 96 hours after treatment. Both fumigant and repellent activities showed a gradual increase in insect mortality and repellency with time of exposure as the concentrations of the essential oils were increased; 100% repellency was achieved at 8µL in the second hour and this remained constant with time. The lowest repellency of 20% was obtained at 2µL. In fumigant toxicity, the highest mortality was 70% recorded at 8µL on the third day of exposure. The results indicated that the acetonic essential oil of dry Eucalyptol and 1R-Alpha-Pinene rich Eucalyptus saligna leaves can be developed into a bioinsecticide for controlling maize weevil as a repellent, toxicant and fumigant candidate.
VL  - 4
IS  - 4
ER  -

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