Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels

Rezaul Haque Ansary; Tomal Roy; Ali Asraf; Sabina Easmin

doi:doi:10.11648/j.nano.20200802.13

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Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels

Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin

Published in American Journal of Nano Research and Applications (Volume 8, Issue 2)

Received: 23 May 2020 Accepted: 10 June 2020 Published: 28 June 2020

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Abstract

Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.

Published in	American Journal of Nano Research and Applications (Volume 8, Issue 2)
DOI	10.11648/j.nano.20200802.13
Page(s)	28-34
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Silk Fibroin, Controlled Release, Hydrogels, Nanoparticles, Drug Delivery

References

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APA Style

Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin. (2020). Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. American Journal of Nano Research and Applications, 8(2), 28-34. https://doi.org/10.11648/j.nano.20200802.13

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ACS Style

Rezaul Haque Ansary; Tomal Roy; Ali Asraf; Sabina Easmin. Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. Am. J. Nano Res. Appl. 2020, 8(2), 28-34. doi: 10.11648/j.nano.20200802.13

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AMA Style

Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin. Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. Am J Nano Res Appl. 2020;8(2):28-34. doi: 10.11648/j.nano.20200802.13

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@article{10.11648/j.nano.20200802.13,
  author = {Rezaul Haque Ansary and Tomal Roy and Ali Asraf and Sabina Easmin},
  title = {Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels},
  journal = {American Journal of Nano Research and Applications},
  volume = {8},
  number = {2},
  pages = {28-34},
  doi = {10.11648/j.nano.20200802.13},
  url = {https://doi.org/10.11648/j.nano.20200802.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20200802.13},
  abstract = {Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.},
 year = {2020}
}

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TY - JOUR
T1 - Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels
AU - Rezaul Haque Ansary
AU - Tomal Roy
AU - Ali Asraf
AU - Sabina Easmin
Y1 - 2020/06/28
PY - 2020
N1 - https://doi.org/10.11648/j.nano.20200802.13
DO - 10.11648/j.nano.20200802.13
T2 - American Journal of Nano Research and Applications
JF - American Journal of Nano Research and Applications
JO - American Journal of Nano Research and Applications
SP - 28
EP - 34
PB - Science Publishing Group
SN - 2575-3738
UR - https://doi.org/10.11648/j.nano.20200802.13
AB - Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.
VL - 8
IS - 2
ER -

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Author Information

Rezaul Haque Ansary

Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
Tomal Roy

Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
Ali Asraf

Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
Sabina Easmin

Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh

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APA Style

Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin. (2020). Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. American Journal of Nano Research and Applications, 8(2), 28-34. https://doi.org/10.11648/j.nano.20200802.13

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ACS Style

Rezaul Haque Ansary; Tomal Roy; Ali Asraf; Sabina Easmin. Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. Am. J. Nano Res. Appl. 2020, 8(2), 28-34. doi: 10.11648/j.nano.20200802.13

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AMA Style

Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin. Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. Am J Nano Res Appl. 2020;8(2):28-34. doi: 10.11648/j.nano.20200802.13

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@article{10.11648/j.nano.20200802.13,
  author = {Rezaul Haque Ansary and Tomal Roy and Ali Asraf and Sabina Easmin},
  title = {Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels},
  journal = {American Journal of Nano Research and Applications},
  volume = {8},
  number = {2},
  pages = {28-34},
  doi = {10.11648/j.nano.20200802.13},
  url = {https://doi.org/10.11648/j.nano.20200802.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20200802.13},
  abstract = {Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.},
 year = {2020}
}

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