Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje

Katja Popovska; Milka Zdravkovska; Milena Petrovska; Azis Pollozhani

doi:doi:10.11648/j.ejpm.20140202.11

| Peer-Reviewed

Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje

Katja Popovska, Milka Zdravkovska, Milena Petrovska, Azis Pollozhani

Published in European Journal of Preventive Medicine (Volume 2, Issue 2)

Received: 28 February 2014 Accepted: 8 April 2014 Published: 10 April 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Backgorund: Acinetobacter baumannii is often referred to as the “Gram-negative methicillin-resistant Staphylococcus aureus”, because it is frequently resistant to antibiotics. Clonal outbreaks of carbapenem-resistant and OXA-23–producing A. baumannii have been reported worldwide. Aim: The goal of this study was to promote the phenomenon of disbalance in endemic hospital ECO system which included increase of carbapenemase-resistant Acinetobacter baumanii on account of reduction of MRSA rate in surgical ICU and it’s clonal relatedness as well as the specific precautions. Material nad Methods: Computer database from 1994 – 2012 from surgical ICU patients in the Clinical Center Skopje was used as basic material for this study. Comparative study indicated 2007/8 as a break point period in which almost a twofold decrease of MRSA rates (from over 80% to 45%) versus increased rates of Acinetobacter baumanii (from 29% to 40%) was observed. In 2011 the very first eight strains of carbapenem resistant (resistant to imipenem and meropenem) A. baumannii, were observed. Disc diffusion and VITEK were used for antibiotic susceptibility testing. Resultes: Three distinct strains were detected by PFEGE and were designated as UKIM01AC-1 (5 strains), UKIM01AC-2 (two strains) and 642/2 (one strain). UKIM01AC-1 representatives were PCR positive for bla (OXA-23-like), in addition to the bla (OXA- 51-like) gene which is intrinsic in Acinetobacter baumannii. All isolated strains belonged to European clone II lineage. Conclusion: This clone dispersed very fast in 2012 and achieved the rate of 61.9%. This implicated changes in infection control precautions.

Published in	European Journal of Preventive Medicine (Volume 2, Issue 2)
DOI	10.11648/j.ejpm.20140202.11
Page(s)	20-24
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Acinetobacter, Carbapenem Resistance, Clonal Distribution, Endemic ECO System

References

[1]	Silvia Munoz-Price and Robert A. Weinstein (2008) Acinetobacter Infection N Engl J Med 1271-1281.
[2]	Bou G, Cervero G, Angeles Dominguez M, Quereda C, Martinez-Beltran J.(2000) Characterization of a nosocomial outbreak caused by multiresistant Acinetobacterbaumannii strain with a carbapenem-hydrolyzing enzyme: high-level carbapenem resistance in A. baumannii is not due solely to the presence of β-lactamases. JClin Microbiol. 3299–3305.
[3]	Ethan Rubinstein and Itxhak Levy (1999) Acinetobacter spp. and other non-fermentative pathogens causing nosocomial bacteremias Clin Microbiol Infect 2S29-2S32
[4]	Allen KD, Green HT (1987) Hospital outbreak of multi-resistant Acinetobacter anitratus: an airborne mode of spread? J Hosp Infect 110–119.
[5]	Wernick, A (1995). Pillows, an unexpected source of acinetobacter. Journal of Hospital 189-199
[6]	Raffaele Zarrilli, Margherita Crispino, Maria Bagattini, Elena Barretta, Anna Di Popolo Maria Triassi and Paolo Villari (2004) Molecular Epidemiology of Sequential Outbreaks of Acinetobacter baumannii in an Intensive Care Unit Shows the Emergence of Carbapenem Resistance; J. Clin. Microbiol 946-953
[7]	Tankovic J, Legrand P, De Gaintes G, Cheminea V, Brun Buisson, Duval (1994) J.Characterization of a hospital outbreak of imipenem resistant Acinetobacter baumannii by phenotypic and genotypic typingmethods. J ClinMicrobiol 2677–2681.
[8]	Lolans K, Rice TW, Munoz-Price LS, Quinn JP (2006) Multicity outbreak of carbapenem-resistant Acinetobacter baumannii isolates producing the carbapenemase OXA-40. Antimicrob Agents Chemother 2941-2945
[9]	Wen-Shyand Hsieh, Nai-Yu Wang, Jou-An Feng, Li Chuan Weng and Hsueh Hsia Wu (2012) Types and Prevalence of Carbapenem-Resistant Acinetobacter calcoacticus-Acinetobacter baumanii complex in Northern Taiwan. J Clin Microbiol 2982–2986.
[10]	Chaline S. Antonio, Patricia R. Neves, Micheli Medeiros and Elsa M. Mamikuza (2011) High prevalence of Carbapenem-Resistant Acinetobacter baumanii Carrying the blaOXA-143 Gene in Brasilian Hospitals. 1322-1233.
[11]	Rodriguez-Bano J, Cisneros JM, Fernandez-Cuenca F (2004) Clinical features and epidemiology of Acinetobacter baumannii colonization and infection in Spanish hospitals. Infect Control Hosp Epidemiol.819–824
[12]	Turton, J. F.(2006) The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii. FEMS Microbiol. Lett. 258:72-77.
[13]	Turton, J. F (2006) Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J. Clin. Microbiol 2974-2976.
[14]	Evans B.A., Hamouda A., Amyes U.K (2013) The rise od carbapenem-resistant Acinetobacter baumanii. University of Manchester, Manchester, UK, 223-38.
[15]	Paul G. Higgins1*, Cathrin Dammhayn1, Meredith Hackel2 and Harald Seifert1. (2009) Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother 1091-1093
[16]	Rea, E. Upshur, R. (2001). Semmelweis revisited: the ethics of infection prevention among health care workers. Canadian Medical Association Journal 1447-1448.
[17]	Charline S. Antonio, Patricia R. Neves, Micheli Medeiros and Elsa M. Mamizuka (2010). High prevalence of carbapenem-resistant Acinetobacter baumanii carrying the bla OXA-143 gene in Brazillian Hospitals. Anim Agent Chemoter 1322-1323
[18]	Wen-Sen-ShyangHsieh, Nai-Yu Wang, Jou-An Feng. Types and prevalence of carbapenem-resistant Acinetobacter calcoaceticus- acinetobacter baumaniii complex in Northern Taiwan (published before print 2013) Antimicrob Agent Chemoter doi.10.1128/AAC.0079-13
[19]	Retailliau HF, Hightower AW, Dixon RE, Allen JR (1979) Acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. J Infect Dis 371-375.
[20]	McDonald LC, Banerjee S, Jarvis WR (1999) NNIS System. Seasonal variation of Acinetobacter spp. Infections reported to the National Nosocomial Infections Surveillance (NNIS) System: 1987–1996 [abstract no 55]. Infect Control Hosp Epidemiol 1133-1137

Cite This Article

Plain Text BibTeX RIS

APA Style

Katja Popovska, Milka Zdravkovska, Milena Petrovska, Azis Pollozhani. (2014). Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje. European Journal of Preventive Medicine, 2(2), 20-24. https://doi.org/10.11648/j.ejpm.20140202.11

Copy | Download

ACS Style

Katja Popovska; Milka Zdravkovska; Milena Petrovska; Azis Pollozhani. Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje. Eur. J. Prev. Med. 2014, 2(2), 20-24. doi: 10.11648/j.ejpm.20140202.11

Copy | Download

AMA Style

Katja Popovska, Milka Zdravkovska, Milena Petrovska, Azis Pollozhani. Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje. Eur J Prev Med. 2014;2(2):20-24. doi: 10.11648/j.ejpm.20140202.11

Copy | Download

@article{10.11648/j.ejpm.20140202.11,
  author = {Katja Popovska and Milka Zdravkovska and Milena Petrovska and Azis Pollozhani},
  title = {Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje},
  journal = {European Journal of Preventive Medicine},
  volume = {2},
  number = {2},
  pages = {20-24},
  doi = {10.11648/j.ejpm.20140202.11},
  url = {https://doi.org/10.11648/j.ejpm.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20140202.11},
  abstract = {Backgorund: Acinetobacter baumannii is often referred to as the “Gram-negative methicillin-resistant Staphylococcus aureus”, because it is frequently resistant to antibiotics. Clonal outbreaks of carbapenem-resistant and OXA-23–producing A. baumannii have been reported worldwide. Aim: The goal of this study was to promote the phenomenon of disbalance in endemic hospital ECO system which included increase of carbapenemase-resistant Acinetobacter baumanii on account of reduction of MRSA rate in surgical ICU and it’s clonal relatedness as well as the specific precautions. Material nad Methods: Computer database from 1994 – 2012 from surgical ICU patients in the Clinical Center Skopje was used as basic material for this study. Comparative study indicated 2007/8 as a break point period in which almost a twofold decrease of MRSA rates (from over 80% to 45%) versus increased rates of Acinetobacter baumanii (from 29% to 40%) was observed. In 2011 the very first eight strains of carbapenem resistant (resistant to imipenem and meropenem) A. baumannii, were observed. Disc diffusion and VITEK were used for antibiotic susceptibility testing. Resultes: Three distinct strains were detected by PFEGE and were designated as UKIM01AC-1 (5 strains), UKIM01AC-2 (two strains) and 642/2 (one strain). UKIM01AC-1 representatives were PCR positive for bla (OXA-23-like), in addition to the bla (OXA- 51-like) gene which is intrinsic in Acinetobacter baumannii. All isolated strains belonged to European clone II lineage. Conclusion: This clone dispersed very fast in 2012 and achieved the rate of 61.9%. This implicated changes in infection control precautions.},
 year = {2014}
}

Copy | Download

TY - JOUR
T1 - Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje
AU - Katja Popovska
AU - Milka Zdravkovska
AU - Milena Petrovska
AU - Azis Pollozhani
Y1 - 2014/04/10
PY - 2014
N1 - https://doi.org/10.11648/j.ejpm.20140202.11
DO - 10.11648/j.ejpm.20140202.11
T2 - European Journal of Preventive Medicine
JF - European Journal of Preventive Medicine
JO - European Journal of Preventive Medicine
SP - 20
EP - 24
PB - Science Publishing Group
SN - 2330-8230
UR - https://doi.org/10.11648/j.ejpm.20140202.11
AB - Backgorund: Acinetobacter baumannii is often referred to as the “Gram-negative methicillin-resistant Staphylococcus aureus”, because it is frequently resistant to antibiotics. Clonal outbreaks of carbapenem-resistant and OXA-23–producing A. baumannii have been reported worldwide. Aim: The goal of this study was to promote the phenomenon of disbalance in endemic hospital ECO system which included increase of carbapenemase-resistant Acinetobacter baumanii on account of reduction of MRSA rate in surgical ICU and it’s clonal relatedness as well as the specific precautions. Material nad Methods: Computer database from 1994 – 2012 from surgical ICU patients in the Clinical Center Skopje was used as basic material for this study. Comparative study indicated 2007/8 as a break point period in which almost a twofold decrease of MRSA rates (from over 80% to 45%) versus increased rates of Acinetobacter baumanii (from 29% to 40%) was observed. In 2011 the very first eight strains of carbapenem resistant (resistant to imipenem and meropenem) A. baumannii, were observed. Disc diffusion and VITEK were used for antibiotic susceptibility testing. Resultes: Three distinct strains were detected by PFEGE and were designated as UKIM01AC-1 (5 strains), UKIM01AC-2 (two strains) and 642/2 (one strain). UKIM01AC-1 representatives were PCR positive for bla (OXA-23-like), in addition to the bla (OXA- 51-like) gene which is intrinsic in Acinetobacter baumannii. All isolated strains belonged to European clone II lineage. Conclusion: This clone dispersed very fast in 2012 and achieved the rate of 61.9%. This implicated changes in infection control precautions.
VL - 2
IS - 2
ER -

Copy | Download

Author Information

Katja Popovska

Institute of Microbiology and parasitology, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Republic of Macedonia
Milka Zdravkovska

Faculty of Medical Sciences, University “Goce Delchev”, Stip, Republic of Macedonia
Milena Petrovska

Institute of Microbiology and parasitology, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Republic of Macedonia
Azis Pollozhani

Institute for Public Health of the Republic of Macedonia, Skopje, Republic of Macedonia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Katja Popovska, Milka Zdravkovska, Milena Petrovska, Azis Pollozhani. (2014). Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje. European Journal of Preventive Medicine, 2(2), 20-24. https://doi.org/10.11648/j.ejpm.20140202.11

Copy | Download

ACS Style

Katja Popovska; Milka Zdravkovska; Milena Petrovska; Azis Pollozhani. Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje. Eur. J. Prev. Med. 2014, 2(2), 20-24. doi: 10.11648/j.ejpm.20140202.11

Copy | Download

AMA Style

Katja Popovska, Milka Zdravkovska, Milena Petrovska, Azis Pollozhani. Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje. Eur J Prev Med. 2014;2(2):20-24. doi: 10.11648/j.ejpm.20140202.11

Copy | Download

@article{10.11648/j.ejpm.20140202.11,
  author = {Katja Popovska and Milka Zdravkovska and Milena Petrovska and Azis Pollozhani},
  title = {Carbapenem Resistant Acinetobacter Baumanii Versus MRSA Isolates in ICU in Clinical Center Skopje},
  journal = {European Journal of Preventive Medicine},
  volume = {2},
  number = {2},
  pages = {20-24},
  doi = {10.11648/j.ejpm.20140202.11},
  url = {https://doi.org/10.11648/j.ejpm.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20140202.11},
  abstract = {Backgorund: Acinetobacter baumannii is often referred to as the “Gram-negative methicillin-resistant Staphylococcus aureus”, because it is frequently resistant to antibiotics. Clonal outbreaks of carbapenem-resistant and OXA-23–producing A. baumannii have been reported worldwide. Aim: The goal of this study was to promote the phenomenon of disbalance in endemic hospital ECO system which included increase of carbapenemase-resistant Acinetobacter baumanii on account of reduction of MRSA rate in surgical ICU and it’s clonal relatedness as well as the specific precautions. Material nad Methods: Computer database from 1994 – 2012 from surgical ICU patients in the Clinical Center Skopje was used as basic material for this study. Comparative study indicated 2007/8 as a break point period in which almost a twofold decrease of MRSA rates (from over 80% to 45%) versus increased rates of Acinetobacter baumanii (from 29% to 40%) was observed. In 2011 the very first eight strains of carbapenem resistant (resistant to imipenem and meropenem) A. baumannii, were observed. Disc diffusion and VITEK were used for antibiotic susceptibility testing. Resultes: Three distinct strains were detected by PFEGE and were designated as UKIM01AC-1 (5 strains), UKIM01AC-2 (two strains) and 642/2 (one strain). UKIM01AC-1 representatives were PCR positive for bla (OXA-23-like), in addition to the bla (OXA- 51-like) gene which is intrinsic in Acinetobacter baumannii. All isolated strains belonged to European clone II lineage. Conclusion: This clone dispersed very fast in 2012 and achieved the rate of 61.9%. This implicated changes in infection control precautions.},
 year = {2014}
}

Copy | Download